There are a few techniques and procedures most of us gloss over for this checklist, but you really ought to consider adding them to your list of procedures.
Everything here comes from the references shown below, with a few comments in blue.
[G450 Airplane Flight Manual §2-04-30]
[AC 120-74B, ¶7.h.(2)(d)] When entering a runway, either for takeoff or when taxiing into LUAW, flightcrews should make their aircraft more conspicuous to aircraft on final behind them and to ATC by turning on all lights, except for landing lights, that highlight the aircraft’s silhouette. Strobe lights should not be illuminated if they will adversely affect the vision of other pilots. At night, and cleared to LUAW, consider lining up slightly to the left or right of the centerline (CL) (approximately 3 feet) to enable a landing aircraft to visually differentiate that your aircraft from the runway lights.
[AC 120-74B, ¶7.h.(2)(e)] Takeoff. Turn on all lights, including landing lights, when takeoff clearance is received, or when commencing takeoff roll at an airport without an operating control tower.
If you are at an airport with ASDE-X, requiring you to operate your Mode-C on all taxiways, you should have your transponder TCAS in standby. This will prevent airborne aircraft with TCAS versions earlier than 7.0 from detecting you on the ground. We are in the habit of just pressing LSK-6R to turn the transponder on. We should be in the habit of also ensuring the TCAS is in TA/RA. If it isn't, Press the TCAS LSK and then LSK-1L.
Try to learn how much power lever you need to keep the spoilers down once armed. I use a full knob width between throttles. That will avoid the ground spoilers fluttering up and down, which can be disconcerting to the passengers.
What if the V-Speeds aren't boxed? The following items are included in the FMS Takeoff Performance Configuration Check: [G450 OM 2B-26-50.3 (page 33)]:
I used "FAT BAG" as a pneumonic.
When aligning the aircraft with the takeoff runway, the PF should note the aircraft heading, the ATC runway clearance, and the runway number on the pavement are all in agreement. For example, “Heading is two four zero, we are cleared on runway two four, and I see two four.” Once the aircraft is aligned with the runway, both pilots should verify the heading agrees with the runway. Why all this fuss? See the case of Comair Flight 5191 for a case where 49 fatalities could have been avoided.
Advisory Circular 120-74B, Parts 91, 121, 125, and 135 Flight Crew Procedures During Taxi Operations, 7/30/12, U.S. Department of Transportation
Gulfstream G450 Airplane Flight Manual, Revision 35, April 18, 2013